The Phospho-MEK1-T292 Monoclonal Antibody (CABP1021) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed and validated for use in Western blot applications, is highly reactive with human samples.MEK1 is a crucial kinase in the MAPK signaling pathway, playing a key role in cell growth, proliferation, and differentiation. Phosphorylation of MEK1 at threonine 292 is known to regulate the activity of MEK1 and downstream signaling events.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MEK1-T292 Monoclonal Antibody
SKU:
CABP1021
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1566
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PRTP G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
C6 treated with Nocodazole, HeLa treated with Nocodazole, C2C12 treated with CIP
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
RRID
AB_2863906
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit mAb (CABP1021) at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180 s.
Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (CABP1021) at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
