Western blot analysis of HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb (CABP1448) at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM), human TNF-α (50ng/ml) and BV-6 (200nM) at 37℃ for 5 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-RIPK1-S166 Monoclonal Antibody (CABP1448) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the phosphorylated form of RIPK1 at serine 166, a critical site for regulating RIPK1 activity in cell survival and death pathways.RIPK1, or Receptor-Interacting Protein Kinase 1, is a key player in the regulation of cell death and inflammation. Phosphorylation of RIPK1 at serine 166 has been shown to influence its function in various cellular processes, including necroptosis, apoptosis, and inflammation. By targeting this phosphorylation site, researchers can gain valuable insights into the role of RIPK1 in disease states such as cancer, neurodegenerative disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-RIPK1-S166 Monoclonal Antibody
SKU:
CABP1448
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC62873
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MWSK LN
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.
Purification Method
Affinity purification
Gene ID
8737
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1-S166 Rabbit mAb (CABP1448) at 1:4000 dilution. HT-29 cells were treated with Z-VAD (50uM), TNF-α (50ng/ml) and BV-6 (200nM) at 37℃ for 5 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
![Anti-Phospho-RIP (S166) [R03-3P4] Monoclonal Antibody (AGMB05050)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276335/680557/anti-phospho-rip-s166-r03-3p4-monoclonal-antibody-agmb05050__54680.1773042490.jpg?c=2 "Anti-Phospho-RIP (S166) [R03-3P4] Monoclonal Antibody (AGMB05050)")
