The Phospho-Tau-S396 Monoclonal Antibody (CABP1028) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to phosphorylated tau at the S396 site in human samples and has been validated for use in Western blot and immunohistochemistry applications.Tau protein is crucial for maintaining the structure and function of neurons, but abnormal phosphorylation of tau can lead to the formation of neurofibrillary tangles, a hallmark of Alzheimer's disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-Tau-S396 Monoclonal Antibody
SKU:
CABP1028
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1573
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YKSP VV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Purification Method
Affinity purification
Gene ID
4137
RRID
AB_2863912
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S396 Rabbit mAb (CABP1028) at 1:1000 dilution. Mouse brain were treated by CIP(20μL/400μL) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
