The Phospho-TP73-Y99 Antibody (CABP0465) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blotting applications.The antibody specifically binds to the phosphorylated Y99 site on the tumor protein p73, allowing for precise detection and analysis in a variety of cell types. Its versatility and reliability make it an essential tool for investigating the role of phosphorylation in p73 signaling pathways, particularly in cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-TP73-Y99 Antibody
SKU:
CABP0465
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P73, CILD47, Phospho-TP73-Y99
Positive Sample:
MCF7 treated with pervanadate
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
70kDa
Observed MW:
80kDa
This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined.
Purification Method
Affinity purification
Gene ID
7161
RRID
AB_2771627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-TP73-Y99 Rabbit pAb (CABP0465) at 1:500 dilution. MCF7 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
