Anti-PKN1 Antibody (CAB0553)

Antibody Name:	Anti-PKN1 Antibody
Antibody SKU:	CAB0553
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, A-549, 293T, HeLa, HepG2, HT-1080, Mouse kidney, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASD AVQS EPRS WSLL EQLG LAGA DLAA PGVQ QQLE LERE RLRR EIRK ELKL KEGA ENLR RATT DLGR SLGP VELL LRGS SRRL DLLH QQLQ ELHA HVVL PDPA ATHD GPQS PGAG GPTC SATN LSRV AGLE KQLA IELK VKQG AENM IQTY SNGS TKDR KLLL TAQQ MLQD SKTK IDII RMQL RRAL QAGQ LENQ AAPD DTQG SPDL GAVE LRIE ELRH HFRV EHAV AEGA KNVL RLLS AAKA PDRK AVSE AQEK LTES NQKL GLLR EALE RRLG ELPA DHPK GRLL REEL AAAS SAAF
Gene ID:	5585
Uniprot:	Q16512
Cellular Location:	Cell membrane, Cleavage furrow, Cytoplasm, Endosome, Midbody, Nucleus, Peripheral membrane protein
Calculated MW:	104kDa
Observed MW:	120kDa

Synonyms:

PKN1, DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1

Background:

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:AGC group; EC 2.7.11.13; Kinase, protein; PKN family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: cleavage furrow; cytoplasm; cytosol; endosome; midbody; nucleoplasm; nucleus Molecular Function:androgen receptor binding; chromatin binding; GTP-Rho binding; histone binding; histone deacetylase binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein kinase activity; protein kinase C binding; protein serine/threonine kinase activity; Rac GTPase binding Biological Process: activation of JNK activity; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of transcription from RNA polymerase II promoter; signal transduction
NCBI Summary:	The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16512
NCBI GenInfo Identifier:	825505
NCBI Gene ID:	5585
NCBI Accession:	BAA05169.1
UniProt Secondary Accession:	Q16512,Q15143, Q504U4, Q8IUV5, Q9UD44, A8K7W5, B2R9R4 B3KVN3,
UniProt Related Accession:	Q16512
Molecular Weight:	66,060 Da
NCBI Full Name:	PKN
NCBI Synonym Full Names:	protein kinase N1
NCBI Official Symbol:	PKN1
NCBI Official Synonym Symbols:	DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
NCBI Protein Information:	serine/threonine-protein kinase N1
UniProt Protein Name:	Serine/threonine-protein kinase N1
UniProt Synonym Protein Names:	Protease-activated kinase 1; PAK-1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	PKN1

	Western blot analysis of extracts of various cell lines, using PKN1 antibody (CAB0553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded mouse brain using PKN1 antibody (CAB0553) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung using PKN1 antibody (CAB0553) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PKN1 antibody (CAB0553) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
PKN1 Antibody (PACO04736)	Human PKN1 (Protein Kinase N1) ELISA Kit (HUES02264)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)