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PKN1 Rabbit Polyclonal Antibody (CAB0553)

SKU:
CAB0553
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-PKN1 Antibody CAB0553
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-PKN1 Antibody CAB0553
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-PKN1 Antibody CAB0553
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-PKN1 Antibody CAB0553
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:PKN1 Rabbit Polyclonal Antibody
SKU:CAB0553
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3).
Sequence:MASD AVQS EPRS WSLL EQLG LAGA DLAA PGVQ QQLE LERE RLRR EIRK ELKL KEGA ENLR RATT DLGR SLGP VELL LRGS SRRL DLLH QQLQ ELHA HVVL PDPA ATHD GPQS PGAG GPTC SATN LSRV AGLE KQLA IELK VKQG AENM IQTY SNGS TKDR KLLL TAQQ MLQD SKTK IDII RMQL RRAL QAGQ LENQ AAPD DTQG SPDL GAVE LRIE ELRH HFRV EHAV AEGA KNVL RLLS AAKA PDRK AVSE AQEK LTES NQKL GLLR EALE RRLG ELPA DHPK GRLL REEL AAAS SAAF
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA; PKN1
Positive Sample:U-251MG,A-549,293T,HeLa,HepG2,HT-1080,Mouse kidney,Mouse spleen
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cleavage furrow, Cytoplasm, Endosome, Midbody, Nucleus, Peripheral membrane protein.
Calculated MW:104kDa
Observed MW:120kDa

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification Method:Affinity purification
Gene ID:5585
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using PKN1 antibody (CAB0553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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