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Anti-PLCH1 Antibody (CAB12903)

SKU:
CAB12903
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
€139 - €419
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Description

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Antibody Name:Anti-PLCH1 Antibody
Antibody SKU:CAB12903
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human PLCH1 (NP_055811.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Rat
Positive Samples:Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human PLCH1 (NP_055811.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LEGN RGKG RAAT SFSL SDVS MLCS DIPD LHST AILQ ESVI SHLI DNVT LTNE NEPG SSIS ALIG QFDE TNNQ ALTV VSHL HNTS VMSG HCPL PSLG LKMP IKHG FCKG KSKS SFLC SSPE LIAL SSSE TTKH ATNT VYET TCTP ISKT KPDD DLSS KAKT AALE SNLP GSPN TSRG WLPK SPTK GEDW ETLK SCSP ASSP DLTL EDVI ADPT LCFN SGES SLVE IDGE SENL SLTT CEYR REGT SQLA SPLK LKYN QGVV EHFQ RGLR NGYC KETL RPSV PEIF NNIQ DVKT QSIS YLAY Q
Gene ID:23007
Uniprot:Q4KWH8
Cellular Location:Cytoplasm, Membrane
Calculated MW:114kDa/115kDa/184kDa/189kDa
Observed MW:175kDa
Synonyms:PLCH1, PLCL3
Background:
UniProt Protein Function:PLCH1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.4.11; Phospholipase

Chromosomal Location of Human Ortholog: 3q25.31

Cellular Component: cytoplasm; intracellular membrane-bound organelle; plasma membrane

Molecular Function:calcium-dependent phospholipase C activity; phosphoinositide phospholipase C activity

Biological Process: inositol phosphate metabolic process; phosphoinositide-mediated signaling

NCBI Summary:PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009]
UniProt Code:Q4KWH8
NCBI GenInfo Identifier:121947010
NCBI Gene ID:23007
NCBI Accession:Q4KWH8.1
UniProt Secondary Accession:Q4KWH8,Q29RV9, Q4KWH9, Q68CN0, Q86XK4, Q9H9U2, Q9UPT3
UniProt Related Accession:Q4KWH8
Molecular Weight:189kDa
NCBI Full Name:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
NCBI Synonym Full Names:phospholipase C eta 1
NCBI Official Symbol:PLCH1  
NCBI Official Synonym Symbols:PLCL3  
NCBI Protein Information:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
UniProt Protein Name:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1
UniProt Synonym Protein Names:Phosphoinositide phospholipase C-eta-1; Phospholipase C-eta-1; PLC-eta-1; Phospholipase C-like protein 3; PLC-L3
Protein Family:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name:PLCH1  
Anti-PLCH1 Antibody (CAB12903)Western blot analysis of extracts of rat brain, using PLCH1 antibody (CAB12903) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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