The PLCH1 Antibody (CAB12903) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the PLCH1 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in cell signaling, cancer research, and other related fields.PLCH1, also known as phospholipase C-like protein 1, plays a crucial role in the regulation of intracellular calcium levels and cellular signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
PLCH1 Antibody
SKU:
CAB12903
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HPE14, PLCL3, PLC-L3, PLC eta 1, PLCH1
Positive Sample:
Rat brain
Cellular Localization:
Cytoplasm, Membrane.
Calculated MW:
189kDa
Observed MW:
175kDa
PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).
Purification Method
Affinity purification
Gene ID
23007
RRID
AB_2759748
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat brain, using PLCH1 Rabbit pAb (CAB12903) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
