The Porcine HIF-1a (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is specifically designed for the precise detection of HIF-1a levels in porcine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an excellent choice for a variety of research applications. HIF-1a is a key transcription factor that plays a critical role in the cellular response to low oxygen levels. It is involved in regulating genes that control processes such as angiogenesis, metabolism, and cell survival.
By studying HIF-1a levels, researchers can gain valuable insights into the mechanisms underlying hypoxia-related conditions such as cancer, ischemic diseases, and inflammatory disorders. Overall, the Porcine HIF-1a ELISA Kit is a valuable tool for researchers looking to explore the role of HIF-1a in various biological processes and diseases, offering reliable and accurate results for their studies.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-104%
85-100%
83-99%
EDTA Plasma (n = 5)
85-105%
82-100%
85-95%
Heparin Plasma (n = 5)
87-99%
82-94%
83-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-98
94
EDTA Plasma (n = 5)
94-104
98
Heparin Plasma (n = 5)
95-104
100
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
