Human HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
The Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is a specialized assay designed for the quantitative detection of HIF-1α levels in various human biological samples. HIF-1α is a key transcription factor that plays a crucial role in the cellular response to hypoxia, regulating the expression of genes involved in critical processes such as angiogenesis, metabolism, and cell survival. Accurate measurement of HIF-1α is essential for studying oxygen homeostasis, cellular adaptation to low oxygen conditions, and the pathophysiology of multiple diseases. Our HIF-1α ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit provides reliable performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Trust Assay Genie's HIF-1α ELISA Kit for accurate and dependable quantification of this important biomarker in your studies.
Product Name:
Human HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
SKU:
AEES00102
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
