Rat HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
The Rat HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is a specialized assay designed to quantitatively measure the levels of HIF-1α in various biological samples. Hypoxia Inducible Factor 1 Alpha (HIF-1α) is a key transcription factor that plays a central role in cellular responses to low oxygen levels (hypoxia). It regulates the expression of genes involved in crucial physiological processes such as angiogenesis, erythropoiesis, glycolysis, and cell survival under hypoxic conditions. Accurate measurement of HIF-1α levels is vital for understanding the cellular adaptation to hypoxia and the molecular mechanisms underlying oxygen homeostasis. The Rat HIF-1α ELISA Kit offered by Assay Genie provides exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control measures, this kit delivers reliable performance, making it an excellent choice for researchers studying the impact of hypoxia on cellular function and disease pathways.
Product Name:
Rat HIF-1alpha (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
SKU:
AEES00438
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
