Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
The Human HIF-1 Alpha (Hypoxia-Inducible Factor-1 Alpha) CLIA Kit is specifically designed for the accurate quantification of HIF-1 Alpha levels in human samples. This kit is highly sensitive and specific, providing reliable and reproducible results for a variety of research applications.HIF-1 Alpha is a key transcription factor that plays a critical role in cellular responses to hypoxia, regulating the expression of genes involved in processes such as angiogenesis, metabolism, and cell survival. Dysregulation of HIF-1 Alpha has been implicated in various diseases, including cancer, ischemic diseases, and inflammatory disorders, making it a valuable biomarker for understanding disease mechanisms and potential therapeutic interventions.
Overall, the Human HIF-1 Alpha CLIA Kit offers a powerful tool for researchers to study the role of HIF-1 Alpha in various physiological and pathological conditions, advancing our understanding of disease processes and facilitating the development of novel treatments.
Product Name:
Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha) CLIA Kit
SKU:
HUES00733
Target:
Human HIF-1 alpha (Hypoxia Inducible Factor 1 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-119
91-103
102-118
Average (%)
110
97
110
1:4
Range (%)
85-98
90-104
86-97
Average (%)
91
96
91
1:8
Range (%)
86-100
84-96
91-102
Average (%)
91
90
97
1:16
Range (%)
95-109
94-107
85-99
Average (%)
101
101
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.08
299.65
789.01
97.83
321.72
833.58
Standard deviation
10.63
32.51
49.31
9.71
22.55
65.27
C V (%)
9.93
10.85
6.25
9.93
7.01
7.83
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed.
