Anti-PRG2 Antibody (CAB2532)

Antibody Name:	Anti-PRG2 Antibody
Antibody SKU:	CAB2532
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-222 of human PRG2 (NP_002719.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 17-222 of human PRG2 (NP_002719.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LHLR SETS TFET PLGA KTLP EDEE TPEQ EMEE TPCR ELEE EEEW GSGS EDAS KKDG AVES ISVP DMVD KNLT CPEE EDTV KVVG IPGC QTCR YLLV RSLQ TFSQ AWFT CRRC YRGN LVSI HNFN INYR IQCS VSAL NQGQ VWIG GRIT GSGR CRRF QWVD GSRW NFAY WAAH QPWS RGGH CVAL CTRG GHWR RAHC LRRL PFIC SY
Gene ID:	5553
Uniprot:	P13727
Cellular Location:	Cytoplasmic vesicle, Secreted, secretory vesicle
Calculated MW:	23kDa/25kDa
Observed MW:	33KDa

Synonyms:

PRG2, BMPG, MBP, MBP1, proMBP

Background:

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
UniProt Protein Details:	Protein type:Vesicle; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q12 Cellular Component: transport vesicle; extracellular region Molecular Function:heparin binding; carbohydrate binding Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode
NCBI Summary:	The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
UniProt Code:	P13727
NCBI GenInfo Identifier:	281185479
NCBI Gene ID:	5553
NCBI Accession:	P13727.2
UniProt Secondary Accession:	P13727,P81448, Q14227, Q6ICT2, A6XMW0, B2R5I1,
UniProt Related Accession:	P13727
Molecular Weight:	25kDa
NCBI Full Name:	Bone marrow proteoglycan
NCBI Synonym Full Names:	proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
NCBI Official Symbol:	PRG2
NCBI Official Synonym Symbols:	MBP; BMPG; MBP1
NCBI Protein Information:	bone marrow proteoglycan; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein
UniProt Protein Name:	Bone marrow proteoglycan
UniProt Synonym Protein Names:	Proteoglycan 2Eosinophil granule major basic protein; EMBP; MBPAlternative name(s):Pregnancy-associated major basic protein
Protein Family:	Myelin basic protein
UniProt Gene Name:	PRG2
UniProt Entry Name:	PRG2_HUMAN

	Western blot analysis of extracts of Mouse liver, using PRG2 antibody (CAB2532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human placenta using PRG2 Rabbit pAb (CAB2532) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
PRG2 Antibody (PACO11460)	Human Bone marrow proteoglycan / PRG2 ELISA Kit
	Human Bone marrow proteoglycan (PRG2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)