Coagulation Protein C Monoclonal Antibody (CAB4532)
The Coagulation Protein C Monoclonal Antibody (CAB4532) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Coagulation Protein C Monoclonal Antibody
SKU:
CAB4532
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC1023
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PC, APC, PROC1, THPH3, THPH4, Coagulation Protein C
Positive Sample:
HT-29, Mouse liver
Cellular Localization:
Endoplasmic Reticulum, Golgi Apparatus, Secreted.
Calculated MW:
52kDa
Observed MW:
52kDa
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
Purification Method
Affinity purification
Gene ID
5624
RRID
AB_2863289
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Coagulation Coagulation Protein C Rabbit mAb (CAB4532) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Protein C Rabbit mAb (CAB4532, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
