Anti-S100A12 Antibody (CAB5328)

Antibody Name:	Anti-S100A12 Antibody
Antibody SKU:	CAB5328
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A12 (NP_005612.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A12 (NP_005612.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTKL EEHL EGIV NIFH QYSV RKGH FDTL SKGE LKQL LTKE LANT IKNI KDKA VIDE IFQG LDAN QDEQ VDFQ EFIS LVAI ALKA AHYH THKE
Gene ID:	6283
Uniprot:	P80511
Cellular Location:	Cell membrane, Cytoplasm, Peripheral membrane protein, Secreted, cytoskeleton
Calculated MW:	10kDa
Observed MW:	Refer to figures

Synonyms:

S100A12, CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.

UniProt Protein Function:	S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoskeleton; cytoplasm; extracellular region; plasma membrane; nucleus; cytosol Molecular Function:protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding Biological Process: neutrophil chemotaxis; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; mast cell activation; cytokine secretion; activation of NF-kappaB transcription factor; positive regulation of inflammatory response
NCBI Summary:	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]
UniProt Code:	P80511
NCBI GenInfo Identifier:	2507565
NCBI Gene ID:	6283
NCBI Accession:	P80511.2
UniProt Related Accession:	P80511
Molecular Weight:
NCBI Full Name:	Protein S100-A12
NCBI Synonym Full Names:	S100 calcium binding protein A12
NCBI Official Symbol:	S100A12
NCBI Official Synonym Symbols:	p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
NCBI Protein Information:	protein S100-A12
UniProt Protein Name:	Protein S100-A12
UniProt Synonym Protein Names:	CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1; Calgranulin-C; CAGC; Extracellular newly identified RAGE-binding protein; EN-RAGE; Migration inhibitory factor-related protein 6; MRP-6; p6; Neutrophil S100 protein; S100 calcium-binding protein A12Calcitermin
UniProt Gene Name:	S100A12
UniProt Entry Name:	S10AC_HUMAN

	Immunohistochemistry of paraffin-embedded human liver damage using S100A12 antibody (CAB5328) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using S100A12 antibody (CAB5328) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Mouse spleen using S100A12 Rabbit pAb (CAB5328) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Rat spleen using S100A12 Rabbit pAb (CAB5328) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Human spleen using S100A12 Rabbit pAb (CAB5328) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-S100A12 Antibody (CAB12499)	Human S100A12 ELISA Kit
	Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit (HUES02349)
	Human Protein S100-A12 (S100A12) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)