Anti-PSMB1 Antibody (CAB1043)

Antibody Name:	Anti-PSMB1 Antibody
Antibody SKU:	CAB1043
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	PC-3, Mouse brain, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RFSP YVFN GGTI LAIA GEDF AIVA SDTR LSEG FSIH TRDS PKCY KLTD KTVI GCSG FHGD CLTL TKII EARL KMYK HSNN KAMT TGAI AAML STIL YSRR FFPY YVYN IIGG LDEE GKGA VYSF DPVG SYQR DSFK AGGS ASAM LQPL LDNQ VGFK NMQN VEHV PLSL DRAM RLVK DVFI SAAE RDVY TGDA LRIC IVTK EGIR EETV SLRK D
Gene ID:	5689
Uniprot:	P20618
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	26kDa
Observed MW:	25kDa

Synonyms:

PSMB1, HC5, PMSB1, PSC5

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.

UniProt Protein Function:	PSMB1: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
UniProt Protein Details:	Protein type:Proteasome complex; Protease; EC 3.4.25.1; Cell cycle regulation Chromosomal Location of Human Ortholog: 6q27 Cellular Component: cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq, Jul 2008]
UniProt Code:	P20618
NCBI GenInfo Identifier:	130853
NCBI Gene ID:	5689
NCBI Accession:	P20618.2
UniProt Secondary Accession:	P20618,Q9BWA8, B5BU76,
UniProt Related Accession:	P20618
Molecular Weight:	26,489 Da
NCBI Full Name:	Proteasome subunit beta type-1
NCBI Synonym Full Names:	proteasome subunit beta 1
NCBI Official Symbol:	PSMB1
NCBI Official Synonym Symbols:	HC5; PSC5; PMSB1
NCBI Protein Information:	proteasome subunit beta type-1
UniProt Protein Name:	Proteasome subunit beta type-1
UniProt Synonym Protein Names:	Macropain subunit C5; Multicatalytic endopeptidase complex subunit C5; Proteasome component C5; Proteasome gamma chain
Protein Family:	Proteasome
UniProt Gene Name:	PSMB1
UniProt Entry Name:	PSB1_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMB1 antibody (CAB1043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using PSMB1 antibody (CAB1043) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate cancer using PSMB1 antibody (CAB1043) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PSMB1 antibody (CAB1043) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PSMB1 antibody (CAB1043) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

PSMB1 Antibody (PACO11529)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)