Anti-SLC4A5 Antibody (CAB10436)

Antibody Name:	Anti-SLC4A5 Antibody
Antibody SKU:	CAB10436
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, U-87MG, SW480, SGC-7901, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DFIF SQHD LAWI DNIL PEKE KKET DKKR KRKK GAHE DCDE EPQF PPPS VIKI PMES VQSD PQNG IHCI ARKR SSSW SYSL
Gene ID:	57835
Uniprot:	Q9BY07
Cellular Location:	Apical cell membrane, Multi-pass membrane protein
Calculated MW:	107-126kDa
Observed MW:	110kDa

Synonyms:

SLC4A5, NBC4, NBCe2

Background:

This gene encodes a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

UniProt Protein Function:	SLC4A5: Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na(+):HCO3(-) stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na(+):HCO3(-) cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH. Belongs to the anion exchanger (TC 2.A.31) family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:anion transmembrane transporter activity; sodium:bicarbonate symporter activity Biological Process: bicarbonate transport; regulation of intracellular pH; renal system process
NCBI Summary:	This gene encodes a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BY07
NCBI GenInfo Identifier:	182691595
NCBI Gene ID:	57835
NCBI Accession:	Q9BY07.2
UniProt Secondary Accession:	Q9BY07,Q32MA7, Q59EQ9, Q8WXD3, Q8WXD7, Q96DS7, Q96DS8 Q9HBU5,
UniProt Related Accession:	Q9BY07
Molecular Weight:	126kDa
NCBI Full Name:	Electrogenic sodium bicarbonate cotransporter 4
NCBI Synonym Full Names:	solute carrier family 4 member 5
NCBI Official Symbol:	SLC4A5
NCBI Official Synonym Symbols:	NBC4; NBCe2
NCBI Protein Information:	electrogenic sodium bicarbonate cotransporter 4
UniProt Protein Name:	Electrogenic sodium bicarbonate cotransporter 4
UniProt Synonym Protein Names:	NBCe2; Solute carrier family 4 member 5
Protein Family:	Electrogenic sodium bicarbonate cotransporter
UniProt Gene Name:	SLC4A5

	Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (CAB10436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat liver using SLC4A5 antibody (CAB10436) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using SLC4A5 antibody (CAB10436) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using SLC4A5 antibody (CAB10436) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)