Anti-PSMD13 Antibody (CAB6956)

Antibody Name:	Anti-PSMD13 Antibody
Antibody SKU:	CAB6956
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, Jurkat, HL-60, SW620, Mouse testis, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PSMD13 (NP_002808.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKDV PGFL QQSQ NSGP GQPA VWHR LEEL YTKK LWHQ LTLQ VLDF VQDP CFAQ GDGL IKLY ENFI SEFE HRVN PLSL VEII LHVV RQMT DPNV ALTF LEKT REKV KSSD EAVI LCKT AIGA LKLN IGDL QVTK ETIE DVEE MLNN LPGV TSVH SRFY DLSS KYYQ TIGN HASY YKDA LRFL GCVD IKDL PVSE QQER AFTL GLAG LLGE GVFN FGEL LMHP VLES LRNT DRQW LIDT LYAF NSGN VERF QT
Gene ID:	5719
Uniprot:	Q9UNM6
Cellular Location:
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

PSMD13, HSPC027, Rpn9, S11, p40.5

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described.

UniProt Protein Function:	PSMD13: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S11 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytosol; extracellular region; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle Molecular Function:structural molecule activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; neutrophil degranulation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; proteasome assembly; protein deubiquitination; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UNM6
NCBI GenInfo Identifier:	317373273
NCBI Gene ID:	5719
NCBI Accession:	Q9UNM6.2
UniProt Secondary Accession:	Q9UNM6,O75831, Q53XU2, Q9UNV3, B3KT15,
UniProt Related Accession:	Q9UNM6
Molecular Weight:	43kDa
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 13
NCBI Synonym Full Names:	proteasome 26S subunit, non-ATPase 13
NCBI Official Symbol:	PSMD13
NCBI Official Synonym Symbols:	S11; Rpn9; p40.5; HSPC027
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 13
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 13
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome regulatory subunit p40.5
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD13

	Western blot analysis of extracts of various cell lines, using PSMD13 antibody (CAB6956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using PSMD13 antibody (CAB6956) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PSMD13 antibody (CAB6956) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PSMD13 Polyclonal Antibody (CAB6956) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using PSMD13 Polyclonal Antibody (CAB6956) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PSMD13 Antibody (CAB12485)

PSMD13 Antibody (PACO11548)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)