The QuickStep Chicken E3 Estriol ELISA Kit from AssayGenie is a reliable and efficient tool designed for the precise quantification of E3 Estriol levels in chicken serum and plasma samples. This ELISA kit offers high sensitivity and accuracy, ensuring consistent and trustworthy results for your research needs.E3 Estriol, a type of estrogen hormone, plays a crucial role in reproductive health and hormonal balance in chickens. Monitoring E3 Estriol levels can provide valuable insights into reproductive cycles, egg production, and overall health in poultry.
This ELISA kit is an essential tool for poultry researchers, veterinarians, and professionals in the agricultural industry looking to study and optimize chicken reproductive health.Trust the QuickStep Chicken E3 Estriol ELISA Kit to deliver reliable and precise measurements of E3 Estriol levels in chicken samples, enabling you to advance your research and improve the health and breeding practices of poultry.
Product Name:
QuickStep Chicken Estriol ELISA Kit
Product Code:
QSES013
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Chicken
Sensitivity:
1.09 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Chicken serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken E3 are added to the micro ELISA plate wells. Chicken E3 in samples (or standards) competes with a fixed amount of Chicken E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Chicken E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Chicken E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.80
28.97
67.98
10.49
28.39
66.14
Standard deviation
0.62
1.23
3.71
0.71
1.49
3.45
CV (%)
6.28
4.23
5.46
6.79
5.26
5.22
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
86-102
93
EDTA plasma(n=8)
95-108
100
Samples were spiked with high concentrations of Chicken E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
