The Quickstep Chicken PG (Progesterone) ELISA Kit is specifically designed for the accurate measurement of progesterone levels in chicken serum, plasma, and tissue samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Progesterone is a key hormone in the reproductive processes of chickens, regulating the menstrual cycle, ovulation, and pregnancy.
Monitoring progesterone levels is crucial for breeding programs, egg production studies, and reproductive health research in poultry.With its user-friendly protocol and reliable performance, the Quickstep Chicken PG ELISA Kit is an essential tool for researchers and veterinarians working with chicken reproductive biology. Ensure the success of your poultry studies with this advanced kit from AssayGenie.
Product Name:
QuickStep Chicken Progesterone ELISA Kit
Product Code:
QSES014
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Chicken
Sensitivity:
0.06 ng/mL
Range:
0.16-10 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Chicken serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken Pg are added to the micro ELISA plate wells. Chicken Pg in samples (or standards) competes with a fixed amount of Chicken Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Chicken Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Chicken Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
0.48
1.45
3.41
0.49
1.38
3.42
Standard deviation
0.03
0.08
0.17
0.03
0.07
0.19
CV (%)
5.25
5.76
4.98
5.57
5.10
5.48
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
90-104
98
EDTA plasma(n=8)
89-102
95
Samples were spiked with high concentrations of Chicken Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
