Anti-R-spondin-1 Antibody (CAB8289)

Antibody Name:	RSPO1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8289
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-263 of human RSPO1 (NP_001033722.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, 22Rv1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-263 of human RSPO1 (NP_001033722.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SRGI KGKR QRRI SAEG SQAC AKGC ELCS EVNG CLKC SPKL FILL ERND IRQV GVCL PSCP PGYF DARN PDMN KCIK CKIE HCEA CFSH NFCT KCKE GLYL HKGR CYPA CPEG SSAA NGTM ECSS PAQC EMSE WSPW GPCS KKQQ LCGF RRGS EERT RRVL HAPV GDHA ACSD TKET RRCT VRRV PCPE GQKR RKGG QGRR ENAN RNLA RKES KEAG AGSR RRKG QQQQ QQQG TVGP LTSA GPA
Gene ID:	284654
Uniprot:	Q2MKA7
Cellular Location:	Secreted
Calculated MW:	21kDa/25kDa/28kDa
Observed MW:	29kDa

Synonyms:

RSPO1, CRISTIN3, RSPO

Background:

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Secreted, signal peptide; Cell development/differentiation; Secreted Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: extracellular space; extracellular region; nucleus Molecular Function:heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding Biological Process: regulation of gene expression; regulation of receptor internalization; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; male meiosis; positive regulation of Wnt receptor signaling pathway Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal
NCBI Summary:	This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:	Q2MKA7
NCBI GenInfo Identifier:	97189599
NCBI Gene ID:	284654
NCBI Accession:	Q2MKA7.1
UniProt Secondary Accession:	Q2MKA7,Q0H8S6, Q14C72, Q5T0F2, Q8N7L5, A2A420,
UniProt Related Accession:	Q2MKA7
Molecular Weight:	21,775 Da
NCBI Full Name:	R-spondin-1
NCBI Synonym Full Names:	R-spondin 1
NCBI Official Symbol:	RSPO1
NCBI Official Synonym Symbols:	RSPO; CRISTIN3
NCBI Protein Information:	R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
UniProt Protein Name:	R-spondin-1
UniProt Synonym Protein Names:	Roof plate-specific spondin-1; hRspo1
Protein Family:	R-spondin
UniProt Gene Name:	RSPO1
UniProt Entry Name:	RSPO1_HUMAN

	Western blot analysis of extracts of various cell lines, using RSPO1 antibody (CAB8289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using RSPO1 antibody (CAB8289) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using RSPO1 antibody (CAB8289) at dilution of 1:100 (40x lens).

ELISA Kits

Human R-spondin-1 / RSPO1 ELISA Kit

Human R-spondin-1 (RSPO1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)