Anti-RAB31 Antibody (CAB3667)

Antibody Name:	Anti-RAB31 Antibody
Antibody SKU:	CAB3667
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human RAB31 (NP_006859.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Mouse lung, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human RAB31 (NP_006859.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMAI RELK VCLL GDTG VGKS SIVC RFVQ DHFD HNIS PTIG ASFM TKTV PCGN ELHK FLIW DTAG QERF HSLA PMYY RGSA AAVI VYDI TKQD SFYT LKKW VKEL KEHG PENI VMAI AGNK CDLS DIRE VPLK DAKE YAES IGAI VVET SAKN AINI EELF QGIS RQIP PLDP HENG NNGT IKVE KPTM QASR RCC
Gene ID:	11031
Uniprot:	Q13636
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Golgi apparatus, Lipid-anchor, phagosome, phagosome membrane, trans-Golgi network, trans-Golgi network membrane
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:	RAB31, Rab22B
Background:

UniProt Protein Function:	RAB31: Belongs to the small GTPase superfamily. Rab family
UniProt Protein Details:	Protein type:G protein, monomeric; G protein; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 18p11.3 Cellular Component: phagocytic vesicle membrane; late endosome; early endosome; trans-Golgi network membrane; phagocytic vesicle Molecular Function:GTPase activity; GDP binding; GTP binding Biological Process: regulated secretory pathway; cellular response to insulin stimulus; receptor internalization; Golgi to plasma membrane protein transport; Rab protein signal transduction
NCBI Summary:	Small GTP-binding proteins of the RAB family, such as RAB31, play essential roles in vesicle and granule targeting (Bao et al., 2002 [PubMed 11784320]).[supplied by OMIM, Jul 2009]
UniProt Code:	Q13636
NCBI GenInfo Identifier:	2500069
NCBI Gene ID:	11031
NCBI Accession:	Q13636.1
UniProt Secondary Accession:	Q13636,Q15770, Q9HC00, B2RBT7,
UniProt Related Accession:	Q13636
Molecular Weight:	194
NCBI Full Name:	Ras-related protein Rab-31
NCBI Synonym Full Names:	RAB31, member RAS oncogene family
NCBI Official Symbol:	RAB31
NCBI Official Synonym Symbols:	Rab22B
NCBI Protein Information:	ras-related protein Rab-31; ras-related protein Rab-22B
UniProt Protein Name:	Ras-related protein Rab-31
UniProt Synonym Protein Names:	Ras-related protein Rab-22B
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB31
UniProt Entry Name:	RAB31_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB31 antibody (CAB3667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using RAB31 antibody (CAB3667). Blue: DAPI for nuclear staining.

Antibodies

Secondary Antibody