The Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is a cutting-edge assay designed for the precise quantitative analysis of TNF-α levels in a range of rabbit biological samples. TNF-α, a pivotal pro-inflammatory cytokine, plays a fundamental role in a variety of cellular functions, from cell proliferation to immune regulation. Its involvement in inflammatory processes, immune responses, and disease pathology has made it a key target for research and therapeutic development. This ELISA kit empowers researchers to accurately determine TNF-α concentrations, providing valuable insights into the role of this cytokine in inflammatory conditions, autoimmune disorders, and infections. By quantifying TNF-α levels, researchers can deepen their understanding of its function in disease mechanisms, supporting the development of targeted treatment strategies and potential therapeutic interventions. Manufactured to the highest standards, the Rabbit TNF-α ELISA Kit from Assay Genie ensures exceptional sensitivity, specificity, and reproducibility, delivering dependable results for research applications. With easy-to-use protocols and robust performance, this kit is an excellent choice for scientists investigating TNF-α biology and its implications in rabbit model studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
