Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit
The Human TNF-alpha (Tumor Necrosis Factor-alpha) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.TNF-alpha is a key cytokine involved in inflammation, immune response, and apoptosis. Dysregulation of TNF-alpha has been associated with various inflammatory diseases, autoimmune disorders, and cancer. By accurately measuring TNF-alpha levels, researchers can gain valuable insights into the pathogenesis of these diseases and develop potential therapeutic strategies.The Human TNF-alpha ELISA Kit from Assay Genie offers researchers a reliable method for detecting and quantifying TNF-alpha levels in human samples, providing a valuable tool for studying the role of TNF-alpha in disease pathophysiology and identifying potential therapeutic targets.
Product Name:
Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit
SKU:
HUES01395
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
