null

Anti-RANGAP1 Antibody (CAB5381)

SKU:
CAB5381
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 9 Anti-RANGAP1 Antibody CAB5381
  • Cell Biology Antibodies 9 Anti-RANGAP1 Antibody CAB5381
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RANGAP1 Antibody
Antibody SKU:CAB5381
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RANGAP1 (NP_002874.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474, HeLa, MCF7, A-549, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RANGAP1 (NP_002874.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PQQR GQGE KSAT PSRK ILDP NTGE PAPV LSSP PPAD VSTF LAFP SPEK LLRL GPKS SVLI AQQT DTSD PEKV VSAF LKVS SVFK DEAT VRMA VQDA VDAL MQKA FNSS SFNS NTFL TRLL VHMG LLKS EDKV KAIA NLYG PLMA LNHM VQQD YFPK ALAP LLLA FVTK PNSA LESC SFAR HSLL QTLY KV
Gene ID:5905
Uniprot:P46060
Cellular Location:Chromosome, Cytoplasm, Cytoplasmic side, Nucleus membrane, Peripheral membrane protein, centromere, cytoskeleton, kinetochore, spindle pole
Calculated MW:63kDa
Observed MW:64kDa
Synonyms:RANGAP1, Fug1, RANGAP, SD
Background:This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RANGAP1: a cytoplasmic GTPase activator for the Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. Homodimerizes. Forms a tight complex in association with RANBP2 and the ubiquitin-conjugating enzyme E2 (UBC9).
UniProt Protein Details:

Protein type:GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; kinetochore; nuclear envelope; nuclear membrane; nuclear pore

Molecular Function:protein binding

Biological Process: negative regulation of protein export from nucleus; protein sumoylation; sister chromatid cohesion

NCBI Summary:This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:P46060
NCBI GenInfo Identifier:1172922
NCBI Gene ID:5905
NCBI Accession:P46060.1
UniProt Secondary Accession:P46060,Q96JJ2,
UniProt Related Accession:P46060
Molecular Weight:63,542 Da
NCBI Full Name:Ran GTPase-activating protein 1
NCBI Synonym Full Names:Ran GTPase activating protein 1
NCBI Official Symbol:RANGAP1  
NCBI Official Synonym Symbols:SD; Fug1; RANGAP  
NCBI Protein Information:ran GTPase-activating protein 1
UniProt Protein Name:Ran GTPase-activating protein 1
Protein Family:RAN GTPase-activating protein
UniProt Gene Name:RANGAP1  
UniProt Entry Name:RAGP1_HUMAN
Anti-RANGAP1 Antibody (CAB5381)Western blot analysis of extracts of various cell lines, using RANGAP1 antibody (CAB5381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-RANGAP1 Antibody (CAB5381)Immunohistochemistry of paraffin-embedded mouse lung using RANGAP1 antibody (CAB5381) at dilution of 1:100 (40x lens).

Related Products