The Rat ADH (Antidiuretic Hormone) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Antidiuretic Hormone in various biological samples. Antidiuretic Hormone, also known as vasopressin, plays a crucial role in regulating water balance, blood pressure, and concentration of urine. Accurate measurement of Antidiuretic Hormone is essential for understanding its physiological functions and its role in various conditions related to water balance and renal function. The Rat ADH ELISA Kit provides exceptional sensitivity and specificity for precise and reliable results. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an invaluable tool for researchers studying hormone regulation and related mechanisms.
Product Name:
Rat ADH (Antidiuretic Hormone) ELISA Kit
SKU:
AEES00442
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
