The Human ADH (Antidiuretic Hormone) ELISA Kit is specifically designed for the precise quantification of ADH levels in human serum, plasma, and cell culture supernatants. This kit is renowned for its exceptional sensitivity and specificity, ensuring accurate and consistent results, thereby making it an invaluable tool for various research applications.ADH, also known as vasopressin, is a vital hormone that regulates water balance in the body by controlling the reabsorption of water in the kidneys. Imbalances in ADH levels can lead to disorders such as diabetes insipidus and syndrome of inappropriate antidiuretic hormone secretion (SIADH). Therefore, measuring ADH levels is crucial for understanding and managing these conditions.With its advanced technology and user-friendly format, the Human ADH ELISA Kit is an essential resource for researchers studying fluid balance disorders, renal function, and other physiological processes influenced by ADH. Trust in this kit to deliver precise and reliable results that will advance your understanding of ADH-related pathways and enhance your research endeavors.
Product Name:
Human ADH (Antidiuretic Hormone) ELISA Kit
SKU:
HUES01508
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
