The Mouse ADH (Antidiuretic Hormone) ELISA Kit is specifically designed for the precise measurement of ADH levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research applications.ADH, also known as vasopressin, is a key hormone involved in regulating water balance in the body by controlling fluid reabsorption in the kidneys. Dysregulation of ADH levels can lead to disorders such as diabetes insipidus and syndrome of inappropriate antidiuretic hormone (SIADH). Understanding ADH levels is crucial for studying these conditions and developing potential treatments.By utilizing the Mouse ADH ELISA Kit, researchers can gain valuable insights into the role of ADH in various physiological and pathological processes, ultimately advancing our understanding of water balance regulation and related disorders in mouse models.
Product Name:
Mouse ADH (Antidiuretic Hormone) ELISA Kit
SKU:
MOES00704
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
