The Rat ANA (Anti-Nuclear Antibody) ELISA Kit from Assay Genie is a reliable and accurate tool for the detection of anti-nuclear antibodies in rat serum samples. This kit is designed with high sensitivity and specificity to ensure precise and reproducible results, making it an ideal choice for a wide range of research applications. Anti-nuclear antibodies are autoantibodies that target components within the cell nucleus and are commonly associated with autoimmune diseases such as lupus and rheumatoid arthritis. The Rat ANA ELISA Kit allows researchers to study the presence and levels of these antibodies in rat models, providing valuable insights into autoimmune disease mechanisms and potential therapeutic targets.
With this kit, researchers can easily and efficiently measure anti-nuclear antibody levels in rat samples, making it a valuable tool for advancing research in autoimmune diseases and other related fields. Trust Assay Genie for high-quality ELISA kits that deliver accurate and reliable results for your research needs.
Product Name:
Rat ANA (Anti-nuclear Antibody) ELISA Kit
SKU:
AEFI00965
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
0.281 ng/mL
Range:
0.469-30 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-95%
88-98%
80-92%
EDTA Plasma (n = 5)
92-103%
88-98%
90-100%
Heparin Plasma (n = 5)
88-102%
87-95%
85-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
94
EDTA Plasma (n = 5)
86-100
93
Heparin Plasma (n = 5)
90-103
97
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
