The Rat AR (Amphiregulin) CLIA Kit is specifically designed for the precise measurement of amphiregulin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for various research purposes.Amphiregulin is a key signaling protein involved in cell growth, differentiation, and tissue repair. Its dysregulation has been implicated in various diseases, including cancer, inflammatory disorders, and wound healing deficiencies. Therefore, monitoring amphiregulin levels is crucial for understanding the underlying mechanisms of these conditions and exploring potential therapeutic strategies.With its user-friendly protocol and reliable performance, the Rat AR CLIA Kit is an invaluable tool for scientists and researchers studying the roles of amphiregulin in health and disease. Get your kit today and advance your research in the field of biomarker discovery and therapeutic development.
Product Name:
Rat AR (Amphiregulin) CLIA Kit
SKU:
RTES00352
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
