The Human AR (Amphiregulin) ELISA Kit is a powerful tool for measuring amphiregulin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it suitable for a wide range of research applications.Amphiregulin is a key growth factor that plays a critical role in cell proliferation, differentiation, and tissue repair. Its dysregulation has been implicated in various diseases, including cancer, inflammatory disorders, and tissue damage. As a result, the Human AR ELISA Kit is an essential tool for studying the biology of amphiregulin and its potential implications in disease mechanisms and therapeutic interventions.Get your Human AR ELISA Kit today and unlock the potential of amphiregulin research in advancing our understanding of human health and disease. Visit our website for more information and to place your order.
Product Name:
Human AR (Amphiregulin) ELISA Kit
SKU:
HUES01483
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
