The Rat Chromogranin B (ChgB) ELISA Kit is specifically designed for the precise measurement of chromogranin B levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it an invaluable tool for a variety of research studies.Chromogranin B is a key protein involved in regulating neurotransmitter release and is found abundantly in neuroendocrine cells. It plays a crucial role in various physiological processes, including hormone secretion, neurotransmission, and immune response modulation.
Dysregulation of chromogranin B has been associated with several diseases, such as neuroendocrine tumors and cardiovascular disorders, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Rat Chromogranin B (ChgB) ELISA Kit offers researchers a reliable and efficient means of quantifying chromogranin B levels in biological samples, enabling in-depth investigations into its function and potential clinical implications.
Product Name:
Rat Chromogranin B (CHGB) ELISA Kit
Product Code:
RTDL00209
Size:
96 Assays
Target:
CHGB
Synonyms:
SCG1, CgB, SgI, Secretoneurin 1, Secretogranin 1
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
