The Rat Gonadotropin-Releasing Hormone (GnRH) ELISA Kit is a reliable and accurate tool for measuring levels of GnRH in rat samples. This kit is specifically designed for use with rat serum, plasma, and tissue homogenates, offering high sensitivity and specificity for precise results. Gonadotropin-releasing hormone is a key regulator of reproductive function, controlling the release of hormones that influence the menstrual cycle, fertility, and sexual development.
Dysregulation of GnRH levels can lead to disorders such as infertility and hormonal imbalances, making it a crucial marker for research in reproductive biology and endocrinology. With its easy-to-use protocol and fast assay time, the Rat GnRH ELISA Kit is suitable for a variety of research applications, providing valuable insights into the role of GnRH in reproductive health and potential therapeutic interventions.
Product Name:
Rat Gonadotropin-Releasing Hormone/GnRH ELISA Kit
SKU:
RTFI00826
Reactivity:
Rat
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
82-97%
86-97%
85-101%
EDTA Plasma (n = 10)
81-100%
83-94%
80-94%
Heparin Plasma (n = 10)
85-104%
90-100%
89-103%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
90-105
90
EDTA Plasma (n = 10)
88-103
94
Heparin Plasma (n = 10)
88-100
98
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
