The GNRH (Gonadotropin-Releasing Hormone) ELISA Kit is a powerful tool for the quantitative measurement of GNRH levels in various biological samples, including serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.GNRH is a critical hormone involved in the regulation of reproductive processes, including the release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary gland. Dysregulation of GNRH signaling has been implicated in disorders such as infertility, polycystic ovarian syndrome, and hypogonadism, making it a key biomarker for understanding and diagnosing these conditions. By accurately measuring GNRH levels, researchers can gain valuable insights into the mechanisms underlying reproductive health and disease, paving the way for new diagnostic tools and therapeutic strategies. With its high sensitivity and reliability, the GNRH ELISA Kit is an indispensable tool for investigating the role of GNRH in physiological and pathological processes.
Product Name:
GnRH (Gonadotropin-Releasing Hormone) ELISA Kit
SKU:
UNES00046
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
