The Rat IL-1R2 ELISA Kit is a specialized assay designed for the quantitative assessment of Interleukin-1 Receptor Type 2 (IL-1R2) levels in a variety of rat biological samples. IL-1R2 plays a crucial role in regulating the biological effects of IL-1, a key pro-inflammatory cytokine involved in diverse cellular processes such as immune response, inflammation, and tissue damage. IL-1R2 acts as a decoy receptor, modulating IL-1 signaling and mitigating its pro-inflammatory effects, thereby influencing immune responses and inflammatory cascades. This ELISA kit offers researchers a reliable method to accurately measure IL-1R2 levels, providing essential insights into the intricate regulatory mechanisms of the IL-1 family. By quantifying IL-1R2 expression, researchers can unravel the nuanced interplay between IL-1 and its receptors, shedding light on the complex immune responses and inflammatory pathways orchestrated by IL-1 signaling. Understanding the role of IL-1R2 is crucial for elucidating its impact on disease processes, immune modulation, and therapeutic interventions targeting IL-1 signaling pathways. With its exceptional sensitivity and specificity, the Rat IL-1R2 ELISA Kit delivers precise and reproducible results, enabling researchers to conduct detailed investigations into the regulatory functions of IL-1R2 in immunological contexts. Manufactured under stringent quality standards, this kit ensures robust performance and user-friendly operation, making it an ideal choice for researchers seeking to explore the intricate roles of IL-1R2 in immune regulation and inflammatory responses.
Product Name:
Rat IL-1R2 ELISA Kit
SKU:
AEES00555
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
