The Rat IL-6 ELISA Kit is a specialized assay intended for the precise quantitative determination of Interleukin-6 (IL-6) levels in a variety of rat biological samples. IL-6 is a pivotal cytokine with multifaceted roles in inflammatory responses, immune regulation, and hematopoiesis, making it a fundamental component in several physiological and pathological processes. Accurate measurement of IL-6 is critical for comprehending its functions in mediating inflammation, immune reactions, and homeostasis, thus offering crucial insights for therapeutic strategies targeting cytokine modulation. Our IL-6 ELISA Kit provides exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible data. Manufactured under rigorous quality control measures, this kit offers robust and consistent performance, coupled with user-friendly protocols, making it an ideal tool for researchers aiming to explore the intricate mechanisms underlying IL-6 activities in various biological contexts.
Product Name:
Rat IL-6 ELISA Kit
SKU:
AEES00297
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
