The Rat IL-4 CLIA Kit is specially designed for the precise measurement of Interleukin-4 (IL-4) levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable tool for a variety of research applications.IL-4 is a crucial cytokine that plays a key role in regulating immune responses, particularly in allergic reactions and autoimmune diseases.
By measuring IL-4 levels, researchers can gain valuable insights into the immune system and its role in various physiological and pathological processes.Whether studying inflammatory conditions, immune responses, or developing new therapeutic strategies, the Rat IL-4 CLIA Kit offers a reliable and efficient method for analyzing IL-4 levels in rat samples. Trust in this kit to deliver high-quality data and drive your research forward.
Product Name:
Rat IL-4 CLIA Kit
SKU:
RTES00014
Target:
Rat IL-4
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-4. You can calculate the concentration of Rat IL-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
86-99
92-106
Average (%)
104
92
98
1:4
Range (%)
88-100
92-107
99-112
Average (%)
93
99
106
1:8
Range (%)
94-108
92-105
97-113
Average (%)
100
97
103
1:16
Range (%)
100-117
83-98
89-103
Average (%)
108
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
94-108
99
Cell culture media (n=5)
98-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.69
64.84
199.89
26.13
66.0
194.83
Standard deviation
2.19
6.52
15.93
2.73
7.75
12.47
C V (%)
8.87
10.06
7.97
10.45
11.74
6.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-4 in samples. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed.
