The Rat IL6 CLIA Kit is a top-of-the-line assay designed for the precise measurement of interleukin-6 (IL6) levels in rat serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and accurate results, making it an excellent tool for a variety of research applications.IL6 is a key cytokine involved in the regulation of the immune response, inflammation, and the acute phase response. Elevated levels of IL6 have been associated with various diseases and conditions, including autoimmune disorders, inflammatory diseases, and cancer.
As such, IL6 serves as a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Trust the Rat IL6 CLIA Kit to provide you with the reliable data you need to advance your research and gain a deeper understanding of the role IL6 plays in health and disease. Order yours today and take your research to the next level.
Product Name:
Rat IL-6 CLIA Kit
SKU:
RTES00015
Target:
Rat IL-6
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-117
94-108
89-102
Average (%)
110
100
94
1:4
Range (%)
91-104
88-105
96-110
Average (%)
98
96
102
1:8
Range (%)
92-105
95-106
88-101
Average (%)
97
101
94
1:16
Range (%)
87-101
93-104
98-111
Average (%)
94
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
105
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.87
112.06
415.55
50.41
118.07
401.3
Standard deviation
5.19
10.16
32.91
6.52
10.63
35.68
C V (%)
9.46
9.07
7.92
12.93
9.0
8.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-6 in samples. No significant cross-reactivity or interference between Rat IL-6 and analogues was observed.
Al-Ghafari,A, B., et al.
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