Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit is a specialized assay designed for the accurate quantitative measurement of Interferon Gamma Induced Protein 10kDa levels in a variety of biological samples. IP-10, also known as CXCL10, is a key chemokine involved in immune responses, particularly regulated by interferon gamma in inflammatory processes. This cytokine plays a crucial role in recruiting immune cells to sites of infection and inflammation and is significant in various immune-related disorders and infections. Accurate measurement of IP-10/CXCL10 levels is vital for elucidating its role in immune responses, autoimmune diseases, and infectious conditions. The Rat IP-10/CXCL10 ELISA Kit from Assay Genie provides exceptional sensitivity and specificity, ensuring reliable and reproducible results. With stringent quality control standards, this kit offers robust performance and user-friendly procedures, making it an excellent choice for cutting-edge research in immunology and inflammatory diseases.
Product Name:
Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
AEES00449
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
