Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Mouse IP-10 (CXCL10) Interferon Gamma-Induced Protein 10kDa ELISA Kit is a powerful tool for quantitative measurement of IP-10 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this ELISA kit provides accurate and reliable results, making it an indispensable tool for researchers in the field.IP-10, also known as CXCL10, is a chemokine induced by interferon gamma and plays a critical role in immune responses, inflammation, and cell migration. Its dysregulation has been implicated in various diseases, including autoimmune disorders, viral infections, and cancer. Therefore, the Mouse IP-10 ELISA Kit is essential for studying the role of IP-10 in these pathologies and developing potential therapeutic interventions.Overall, the Mouse IP-10 Interferon Gamma-Induced Protein 10kDa ELISA Kit from AssayGenie offers a reliable and accurate method for quantifying IP-10 levels in mouse samples, facilitating groundbreaking research in immunology, oncology, and beyond.
Product Name:
Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
MOES00642
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
86-99
89-106
90-103
Average (%)
93
97
97
1:4
Range (%)
95-111
84-95
98-112
Average (%)
101
89
103
1:8
Range (%)
99-113
81-95
93-110
Average (%)
106
87
100
1:16
Range (%)
94-109
86-100
91-105
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
86-98
93
Cell Culture Media (n=8)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.97
187.52
969.08
107.35
183.33
938.75
Standard deviation
7.29
8.61
61.54
8.86
15.33
71.06
C V (%)
6.69
4.59
6.35
8.25
8.36
7.57
Uniport ID:
P17515
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed
