Rat MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
The Rat MCP-1 (Monocyte Chemotactic Protein-1) CLIA Kit is a highly sensitive assay designed for the precise measurement of MCP-1 levels in rat samples. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.MCP-1 is a key chemokine responsible for recruiting monocytes to sites of inflammation, playing a crucial role in immune responses and inflammatory processes. Elevated levels of MCP-1 have been associated with various diseases, including atherosclerosis, rheumatoid arthritis, and kidney disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its high sensitivity and specificity, the Rat MCP-1 CLIA Kit is an essential tool for researchers looking to investigate the role of MCP-1 in disease pathogenesis and study potential treatment options. Its user-friendly format and reliable performance make it a valuable asset in the field of biomedical research.
Product Name:
Rat MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
SKU:
RTES00392
Target:
Rat MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
101-116
85-99
Average (%)
94
107
91
1:4
Range (%)
87-101
89-104
94-105
Average (%)
93
96
99
1:8
Range (%)
99-115
91-103
96-111
Average (%)
107
97
102
1:16
Range (%)
92-106
102-114
95-110
Average (%)
98
108
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.17
177.62
990.85
96.95
186.68
909.39
Standard deviation
10.89
16.63
64.8
9.03
13.63
63.48
C V (%)
10.98
9.36
6.54
9.31
7.3
6.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed.
