Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
The Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit is specifically designed for the quantitative measurement of MCP-1 levels in various biological samples obtained from mouse models. MCP-1 is a crucial chemokine involved in immune responses, inflammation, and the recruitment of monocytes to sites of injury or infection, making it a key player in the regulation of inflammatory processes and immune cell functions. Assay Genie's Mouse MCP-1 ELISA Kit offers high sensitivity and specificity, allowing for accurate and reproducible detection of MCP-1 levels. With stringent quality control measures and validated protocols, this kit ensures robust performance and ease of use, making it an excellent choice for researchers investigating the roles of MCP-1 in inflammation, immune responses, and related diseases. Trust Assay Genie's Mouse MCP-1 ELISA Kit for precise quantification of MCP-1 levels in your research endeavors.
Product Name:
Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
SKU:
AEES00199
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
