Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
The Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit is a specialized tool designed for the quantitative measurement of MCP-1 levels in various rat biological samples. Monocyte Chemotactic Protein 1, also known as CCL2, plays a vital role in immune responses as a potent chemoattractant for monocytes, memory T cells, and dendritic cells. It is involved in the regulation of immune cell migration to sites of inflammation and injury, thus contributing to the immune defense and inflammatory processes in the body. This ELISA kit enables researchers to accurately detect and quantify MCP-1 levels in rat samples, providing valuable insights into the immune response, inflammation, and various disease conditions where MCP-1 is involved. With exceptional sensitivity and specificity, this assay ensures reliable and reproducible results. Manufactured to high quality standards, the Rat MCP-1 ELISA Kit offers robust performance and ease of use, making it an ideal choice for research applications focused on immune responses and inflammatory pathways in rat models.
Product Name:
Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
SKU:
AEES00485
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
