SynonymsM2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
ReactivityRat
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
RTDL00842
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11980
Sensitivity:
0.35ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
M2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
The Rat Pyruvate Kinase Muscle (PKM2) ELISA Kit is specifically designed for the precise measurement of PKM2 levels in rat muscle tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.PKM2 is an essential enzyme that plays a key role in glycolysis and is closely linked to cancer metabolism. Its expression in muscle tissue can provide valuable insights into cellular metabolism and energy production, making it a valuable biomarker for studying metabolic disorders and potential therapeutic interventions.
With easy-to-follow instructions and fast assay procedures, the Rat Pyruvate Kinase Muscle (PKM2) ELISA Kit is a reliable tool for researchers looking to investigate PKM2 levels in rat muscle tissue samples with precision and efficiency.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
