The Rat TSH (Thyroid Stimulating Hormone) CLIA Kit is a highly reliable and sensitive assay designed for the precise measurement of TSH levels in rat serum, plasma, and other biological samples. This kit ensures accurate and reproducible results, making it a valuable tool for research in endocrinology and thyroid-related studies.TSH is a critical hormone that plays a key role in regulating the thyroid gland's function and controlling metabolism in the body. Changes in TSH levels can indicate thyroid dysfunction and various thyroid-related disorders, making it an essential biomarker for studying these conditions and developing potential treatments.
This Rat TSH CLIA Kit provides researchers with a powerful tool to investigate the role of TSH in various physiological and pathological processes, offering valuable insights into thyroid function and potential therapeutic interventions. Trust in the accuracy and precision of this kit for your research needs.
Product Name:
Rat TSH (Thyroid Stimulating Hormone) CLIA Kit
SKU:
RTES00554
Target:
Rat TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
90-103
95-107
Average (%)
98
97
101
1:4
Range (%)
99-111
97-112
99-117
Average (%)
106
105
107
1:8
Range (%)
99-113
97-111
94-105
Average (%)
105
103
100
1:16
Range (%)
99-112
87-99
87-100
Average (%)
106
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
3.05
8.07
1.01
2.91
7.8
Standard deviation
0.13
0.36
0.86
0.1
0.33
0.72
C V (%)
12.26
11.8
10.66
9.9
11.34
9.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSH in samples. No significant cross-reactivity or interference between Rat TSH and analogues was observed.
