Anti-RBFOX3 Antibody (CAB15959)
- SKU:
- CAB15959
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-RBFOX3 Antibody |
| Antibody SKU: | CAB15959 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RBFOX3 (NP_001076045.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RBFOX3 (NP_001076045.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEKK KMVT QGNQ EPTT TPDA MVQP FTTI PFPP PPQN GIPT EYGV PHTQ DYAG QTGE HNLT LYGS TQAH GEQS SNSP STQN GSLT TEGG AQTD GQQS QTQS SENS ESKS TPKR LHVS NIPF RFRD PDLR QMFG QFGK ILDV EIIF NERG SKGF GFVT FENS ADAD RARE KLHG TVVE GRKI EVNN ATAR VMTN KKMV TPYA NGWK LSPV VGAV YGPE LYAA |
| Gene ID: | 146713 |
| Uniprot: | A6NFN3 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 33kDa/35kDa |
| Observed MW: | 54kDa |
| Synonyms: | FOX-3, FOX3, HRNBP3, NEUN, NeuN, RBFOX3 |
| Background: | This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | NeuN: RNA-binding protein that regulates alternative splicing events. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA processing; DNA-binding Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: cytoplasm; perikaryon; nucleus Molecular Function:DNA binding; RNA binding; nucleotide binding Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome |
| UniProt Code: | A6NFN3 |
| NCBI GenInfo Identifier: | 322510139 |
| NCBI Gene ID: | |
| NCBI Accession: | A6NFN3.4 |
| Molecular Weight: | 46-55kDa |
| NCBI Full Name: | RNA binding protein fox-1 homolog 3 |
| UniProt Protein Name: | RNA binding protein fox-1 homolog 3 |
| UniProt Synonym Protein Names: | Fox-1 homolog C |
| UniProt Gene Name: | RBFOX3 |
| UniProt Entry Name: | RFOX3_HUMAN |
 | Western blot analysis of extracts of U-251MG cells, using RBFOX3 antibody (CAB15959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody (CAB15959) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using NeuN antibody (CAB15959) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody (CAB15959) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using RBFOX3 Polyclonal Antibody (CAB15959) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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