The Recombinant AGO2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110231) against AGO2. Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of K562, A549, Mouse brain, PC-12. The target is localized to the cytoplasm, p-body, nucleus, Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
Product Name:
Recombinant AGO2 Monoclonal Antibody
SKU:
AGEL5550
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
AGO, AGO2, Argonaute, Argonaute 2, CASC, CASC7, EIF2C, EIF2C2, LINC, LINC00980, PPD, protein argonaute, protein argonaute-2, Q10
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human AGO2 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110231
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
AGO2
Conjugation:
Unconjugated
Observed MW:
97 kDa
Calculated MW:
97 kDa
Recommended Dilution:
IHC 1:2000-10000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q9UKV8
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
