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Recombinant APE1 Monoclonal Antibody (AGEL6718)

Datasheet MSDS
SKU AGEL6718
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IHC, IP
Host Species Rabbit
Clonality Monoclonal
Clone C121413
Conjugate Unconjugated
€459 - €639

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Description

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Recombinant APE1 Monoclonal Antibody

The Recombinant APE1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121413) against APE1. APEX encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC and IP applications. It has been validated by WB analysis of HEK-293, HepG2, NIH/3T3, RAW264.7, IHC analysis of Human ovarian cancer, Mouse liver, Rat liver and IP analysis of HEK-293 cells extracts. The target is localized to the cytoplasm, nucleus.

Product Name:Recombinant APE1 Monoclonal Antibody
SKU:AGEL6718
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:Ape, Apex, Apex1, Ref, Ref1
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant human APE1 fragment
Applications:WBIHCIP
Clonality:Recombinant Monoclonal
Clone No:C121413
Host:Rabbit
Isotype:Rabbit IgG
Target:APE1
Conjugation:Unconjugated
Observed MW:37 kDa
Calculated MW:35 kDa
Recommended Dilution:WB 1:1000-5000; IHC 1:200-1000; IP 1:50-100
UniProt:P28352
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunohistochemistry of paraffin-embedded Human ovarian cancer using APE1 Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunohistochemistry of paraffin-embedded Mouse liver using APE1 Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunohistochemistry of paraffin-embedded Rat liver using APE1 Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunoprecipitation analysis using anti-APE1 Monoclonal Antibody. Western blot was performed from the immunoprecipitate using APE1 Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: APE1 Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:37 kDa Calculated-MW:35 kDa
Product ImageWestern Blot with APE1 Monoclonal Antibody at dilution of 1:5000. Lane 1: HEK-293, Lane 2: HepG2, Lane 3: NIH/3T3, Lane 4: RAW264.7 Observed-MW:37 kDa Calculated-MW:35 kDa
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