The Recombinant DNAJC6 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121675) against DNAJC6. DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of K562, Mouse liver(negative?control),?Mouse brain, Rat brain and IHC analysis of Human cerebrum, Human liver (negative tissue), Mouse cerebrum, Rat cerebrum. The target is localized to the cytoplasm, postsynaptic density.
Product Name:
Recombinant DNAJC6 Monoclonal Antibody
SKU:
AGEL6980
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
DNAJC, DNAJC6, KIAA, KIAA0473
Reactivity:
Human, Mouse, Rat
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C121675
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
DNAJC6
Conjugation:
Unconjugated
Observed MW:
115 kDa
Calculated MW:
100 kDa
Recommended Dilution:
WB 1:1000; IHC 1:200-400
UniProt:
O75061
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
