The Recombinant ERK5 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170453) against ERK5. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF, IP and ELISA applications. It has been validated by WB analysis of Hela. The target is localized to the cytoplasm, nucleus.
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q13164
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant ERK5 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cell lysate. Observed-MW:115 kDa Calculated-MW:88 kDa